WebThe h-CLAT method is an in vitro test that quantifies changes of cell surface marker expression (CD54 and CD86) following 24 hour exposure to a test item using a human monocytic leukaemia cell line (THP-1) that … WebJul 1, 2024 · The criteria for h-CLAT (CD86 ≥ 150% and/or CD54 ≥ 200%) were used to evaluate the results according to the protocol of h-CLAT (TG442E). The statistical significance of differences in the RFIs of CD86 and CD54 between LPS-treated THP-1 cells and LPS + PMB-treated THP-1 cells was evaluated using paired Student's t-tests (p …

The relationship between CD86/CD54 expression and THP-1 …

Webvation test (h-CLAT) to measure the elevated surface expression of CD86 and CD54 molecules on THP-1 cells by flow cytometry after 24 hr exposure to a test chemical (Sakaguchi et al., 2006; Ashikaga et al., 2010). The h-CLAT exhibited an overall accuracy of 82% to the LLNA data of structurally unrelated 106 chemicals WebDec 1, 2024 · The data used from each source is as follows; the EC 3 inferred from the LLNA, the maximum peptide depletion observed in the DPRA, and dose-response metrics generated as part of the KeratinoSens™ (ARE-Nrf2 EC1.5), h-CLAT (CD86 EC150 and CD54 EC200) and U-SENS™ (CD86 EC200) (Fig. 2, grey boxes). clms football

File extension H86 - Simple tips how to open the H86 file.

Websure to sensitizers. h-CLAT is measures CD86 and CD54 expression on THP-1 cells that serve as a dendritic cell surrogate (Ashikaga et al., 2006, 2010; Sakaguchi et al., 2006). Cells were treated ... WebWe developed a skin sensitization test system based on the markers and criteria of the human cell line activation test (h-CLAT), which combines THP-1 cells as a surrogate for DCs and keratinized... WebDendritic cells were incubated with vaccine microparticles, blank microparticles, and vaccine solution for 16 h. As shown in Figure 5, vaccine microparticles induced the expression of … clms handbook 2021