WebReagent A100, 500 ml . 910-0003 . A reagent for lysis and disagregation of cells.. Reagent B, 500 ml . 910-0002 . A stabilizing buffer . Reagent C, 500 ml . 910-0200 . A reagent used for single cells suspensions . CD with NucleoView v. 2.x . 950-0003 . PC software used for handling of the obtained cell counts . WebNov 8, 2024 · Briefly, the cell–microcarrier suspension was diluted in a 1:3 ratio with reagent A100 (lysing agent) and then reagent B (stabilising agent). The resulting suspension was then loaded onto a Via‐1 cassette containing acridine orange and DAPI. Based on cell counts, the fold increase was calculated using the equation below: ...
10 equipment and accessories – ChemoMetec NC-100 User Manual
WebK2014-KT-A100/A500. ... -20 ℃, avoid repeated freeze-thaw cycles, 12 months. Materials & Reagents Required but Not Included: Manufacturer. Materials. Catalog# bioGenous TM. Primary Tissue Storage Solution. K601005. bioGenous TM Epithelial Organoid Basal Medium. B213151. bioGenous TM. Organoid Dissociation Solution. E238001. bioGenous … WebReagent A100 is an acidic aqueous solution of surfactant and inorganic acid. The pH value is in the range of 1.00 – 1.50. Reagent A100 is used for preparation of nuclei from animal cell cultures and for detaching adherent cell lines and disaggregation of cell clusters. … literally vs literary
Reagent A100 Lysis Buffer ChemoMetec Bioz
WebFor example, to 100 µl of cell suspension add 100 μl of Reagent A100. Afterwards add one volume of Reagent B to the mixture of cell suspension and Reagent A100. To 200 µl of … WebFor example, to 100 µl of cell suspension add 100 μl of Reagent A100. Afterwards add one volume of Reagent B to the mixture of cell suspension and Reagent A100. To 200 µl of the mixture of cell suspension and Reagent A100 add 100 μl of Reagent B and mix by pipetting i.e. Cell Sample: Reagent A100: B, should be mixed in a ratio of 1:1:1. WebReagents, A through F. Reagents, B. ALDRICH Basolite® A100, produced by BASF. PRODUCT AVAILABILITY: Did you know you can view a product's availability right on the … literally vs figuratively examples